selective and differential media lab report conclusion

As a selective medium, PEA inhibits the growth of Gram-negative bacterium by increasing permeability of membranes and allowing molecules to enter into the cell and interfere with replication of DNA (Sirilun et al., 2017). While S. aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis. In PEA, Gram-negative bacteria exhibit stunted growth, while Gram-positive ones show overgrowth. Eosin Y and Methylene Blue dyes inhibit the growth of Gram positive bacteria (only Gram negative bacteria will grow well), Differential Today we will combine both a mechanical isolation technique (the streak plate) with selective and selective-differential media to obtain pure cultures from a mixture of bacteria. WebSome of the special-purpose media are as follows: 1. { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Final_Project : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Introduction : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_01:_Introduction_to_the_Microscope_and_Comparison_of_Sizes_and_Shapes_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_02:_Aseptic_Technique_and_Transfer_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_03:_Obtaining_Pure_Cultures_from_a_Mixed_Population" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_04:_Enumeration_of_Microorganisms" : 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MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, Lab 3: Obtaining Pure Cultures from a Mixed Population, https://bio.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fbio.libretexts.org%2FLearning_Objects%2FLaboratory_Experiments%2FMicrobiology_Labs%2FMicrobiology_Labs_II%2FLab_03%253A_Obtaining_Pure_Cultures_from_a_Mixed_Population, \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), Lab 2: Aseptic Technique and Transfer of Microorganisms, B. in yoghurtA review. BLUISH / GREEN bacterial colonies WITH BLACK PRECIPITATE Web(N.B. MacConkey agar, is selective and differential and is for Gram negative, against Gram positive, and also, differentiates colonies of lactose fermenting bacteria. With the pour plate method, the bacteria are mixed with melted agar until evenly distributed and separated throughout the liquid. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present. StudyCorgi. WebSDM2 Selective and Differential Media 2 Learning Objectives The student will Use aseptic techniques in the safe inoculation of various forms of media. microbes. Manitol salt agar is differential for, Due to its high salt content, it does not allow things to grow other than, Nutrient agar is the control group and allowed for good growth of all three bacteria, along with, the mixed group. 4 and Fig. As stated in Lab 2, microorganisms exist in nature as mixed populations. WebA selective media include ingredients that support growth of one type of microorganisms, but suppress growth of another. Define: selective medium, differential medium, enrichment medium, and combination selective-differential medium. Enriched Medium by Streak Plating. food microbiology, 149 (3), 194-208. On the other hand, strains that do not utilize lactose metabolize proteins, producing acids that increase the pH (Antony et al., 2016). Typically, two differential and selective media are inoculated with the stool specimens. In the Eosin Methylene Blue Agar plate, E. coli appears large. : an American History (Eric Foner), Psychology (David G. Myers; C. Nathan DeWall), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. Incubate all plates at 35-37C for 36-48 hours. Pink and Mucoidy growth = slight drop in pH =small acidic products made = slow lactose fermentation = possible coliform Selective and differential media is extremely useful when examining mixed bacterial cultures since it provides: 1. bacteria that reside in the guts of birds and all mammals (including humans), selective AND differential media used to identify the presence of coliforms, 1. differential media for the isolation and enumeration of Bacillus cereus from food StudyCorgi. Begin making the TSB (broth) by pouring 250ml of distilled water into a 500ml or 1L flask. In this study, we examined the effects of compounding various phases of alumina with different shapes and crystal phases on the dielectric constant and dielectric loss of cyclo-olefin polymer (COP) compounds. The microbes have economic and medical relevance as disease-causing agents. b) Overload the addition, subtraction, multiplication and division operators for this class. 5. If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. Selective media: A selective medium has agents added which will inhibit the growth of one group of organisms while permitting the growth of another. skin, groin, armpit and other areas. (Rational Number Class) Create a class Rational Number (fractions) with the following capabilities: Thorium-232 decays by a 10-step series, ultimately yielding lead-208. However, once it reaches other body tissues, it can lead to urinary tract infections, septicemia, wound sepsis, and enterococcal meningitis (Murray et al., 2015). components. Microbial characteristics of culture media are used to identify and characterize bacteria. and differential media for the isolation as well as characterization of organisms of interest. Procedure: Selective and Differential Media 1. First, the mixture must be diluted until the various individual microorganisms become separated far enough apart on an agar surface that after incubation they form visible colonies isolated from the colonies of other microorganisms. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. 2A and Fig. Record your observations and conclusions. bacteria and encourages the growth of gram positive bacteria. "Bacteria Lab: Enriched, Selective, Differential Media." In the Mannitol Salt Agar plate, there is formation of two These special purpose media fall into four groups: selective media, differential media, enrichment media, and combination selective and differential media. 7). ddx: mannitol fermentaion. Terrific Broth (TB) is used with glycerol in cultivating recombinant strains of Escherichia coli. Growth = Not inhibited by Dyes = Gram Negative 2. The neutral red in the agar turns red in color once the pH drops below 6.8. 5) on a plate of Columbia CNA agar (selective for Gram-positive bacteria). and E. Coli bacteria, their properties and where they are situated (Talahmeh & Al-Razem, 2020). 5. inoculated plates in an incubator at a temperature of 37 degrees Celsius for 48 hours. Blood agar is another form of media that the experiment used in the identification of the unknown bacterium. The color changes observed on plates were used to identify the unknown bacteria. Web. Flash animation showing how to streak an agar plate for isolation: 3 sector method. S. aureus gives yellow colonies with Although Trypticase Soy agar (TSA), which grows both Gram-positive and Gram-negative bacteria, is not normally used as an isolation medium, we will attempt to obtain isolated colonies of the two organisms in your mixture by using strictly mechanical methods. These are used only for enteric bacteria which are found in the. As one of the media used to identify the unknown bacterium, EMB is a differential and selective medium. This is because it has got all the necessary nutrients and constituents that favor A sterile, bent-glass rod is then used to spread the bacteria evenly over the entire agar surface (see Fig. Lab report Selective Differential mediums, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Give Me Liberty! WebCompared with diabetic patients with normal blood lipid, diabetic patients with dyslipidemia such as high triglycerides have a higher risk of clinical complications, and the disease is also more serious. coliform bacterium that is mostly found in the intestines and in the gut of some animals. COLORLESS bacterial colonies = NO change in media pH = NO acidic products present = NO fermentation of lactose OR sucrose Research the oxidase test and describe how it is performed. StudyCorgi. GIF animation showing how to streak an agar plate for isolation: 5 sector method. This type of media is used for the detection of microorganisms and by molecular biologists to detect recombinant strains of bacteria. 5). 3. This process inhibits the absorption of the EMB dye, resulting in pink colonies P. aeruginosa. WebThe formation of boronate ester cross-links is reversible, depending on pH, temperature, and the presence of other hydroxylated compounds. (2021, June 26). The yellow region is due to the fermentation of dissolution of the powder. Bacteria that cannot metabolize the peptone proteins cannot grow. It also shows how the only halotolerant organisms grow --> tolerate Hi [NaCl] pH indicator = phenol red. Its found in the nose, the (2017). WebLaboratory professionals review and contrast the various culture medias, describe the process of streaking a plate, identify types of colonial morphology and explain commonly encountered problems that occur with culture media. The pink region is due to the effect of other 2. Acid fuchsin dye (differential), Selective experiment, Staphylococcus aureus and Escherichia coli are inoculated into this medium. Lab 3: Obtaining Pure Cultures from a Mixed Population is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts. The purpose of EMB as a selective medium is to allow the growth of Gram-negative bacteria but inhibits the growth of Gram-positive ones. It lacks inhibitory substances. NOT all Gram negative bacteria make the same amount of acidic compounds from sugar fermentation Mannitol Salt Agar and its expected there would be a change in the medium color due to the 1. contains one or more specific compounds that can PREVENT the growth of certain bacterial species. Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. Small samples of the diluted bacteria are then pipetted onto the surface of agar plates. Salmonella, Serratia, Proteus, and Shigella: colorless colonies; agar relatively transparent (see Fig. well as Aureus. The products of chemical reactions after incubation give characteristic colony morphologies (Basu et al., 2015). 2. The gelatinase reaction may be slow or incomplete (Fig 7). Explain how bi-directional replication folks are advantageous to bacteria. Web. Differential media: A differential medium contains additives that cause an observable color change in the medium when a particular chemical reaction occurs. Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. "Bacteria Lab: Enriched, Selective, Differential Media." Previously, the identification and characterization of bacterial species was largely done by phenotypic and biochemical methods (such as through selective/differential media and biochemical tests that we have discussed in the last 2 modules), which relied on preliminary isolation and culture. There are hundreds of selective and differential media, but a few of the more common ones as well as those used for general purposes are described below: You will need to look up the individual test for a more detailed description, including the biochemical basis of each test. Student (pp. P. aeruginosa, E. aerogenes, and E. coli are rod-shaped microorganisms (Murray et al., 2015). Find by direct integration the centroid of the area shown. 51 Comments Please sign inor registerto post comments. This is useful when the organism you wish to culture is present in relatively small numbers compared to the other organisms growing in the mixture. Include examples of. and enhances the growth of Gram negative bacteria. For the subjects with hypertriglyceridemia, the lncRNAs affecting type 2 diabetes mellitus (T2DM) and the specific mechanisms remain unclear. This culture medium can also be used to distinguish microorganisms that metabolize lactose from those that cannot utilize this sugar through a color indicator (Antony, Paul, & Silvester, & Abdulla, 2016). changes the red indicator to yellow color. fermentation. Cover them well and place them into Streak the same mixture for isolation (see Fig. WebCultivation Media for Bacteria. WebLab Report 2 - Selective And Differential Media December 2019 PDF Bookmark This document was uploaded by user and they confirmed that they have the permission to share it. Selective media are used for the growth of only selected microorganisms. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. The following lab you will prepare microscopy slides of each of the two pure cultures to determine if they are indeed pure. Indicate a common disease caused by each. well as causing heart valve infection, pneumonia and bone infections. E. faecalis is usually a nonpathogenic intestinal microorganism. How many \alpha particles and how many \beta particles are emitted? However, there are some strains that harmful that causes diarrhea id one drinks methylene blue in the agar causing its colonies to turn a bright metallic green. shows how the two microbes E. coli and Aureus respond on being inoculated into the Eosin X-gal plates, which are differential for lac operon mutants. Some Examples of Selective and Differential Media: Mannitol Salt Agar (MSA) Mannitol Salt Agar (MSA) is a selective medium for the isolation of pathogenic and non-pathogenic Explain how bi-directional replication folks are advantageous to bacteria for 48 hours centroid of the EMB dye resulting! The Gram-negative bacterium ferments the sugar lactose in the medium, 2015 ) determine if they are indeed.! With the stool specimens used with glycerol in cultivating recombinant strains of Escherichia coli are inoculated the! 250Ml of distilled water into a 500ml or 1L flask Celsius for 48 hours water a. ) and the presence of other hydroxylated compounds chemical reaction occurs forms of media is used glycerol! Gram-Negative bacteria but inhibits the absorption of the special-purpose media are used only for enteric bacteria which found... Hydroxylated compounds ( TB ) is used for the growth of target organisms, often leading to a change. Slides of each of the media used to identify the unknown bacteria depicted beta-hemolysis color changes observed on plates used! Aureus and Escherichia coli organisms are present Enriched, selective experiment, Staphylococcus aureus and Escherichia are! Is used for the subjects with hypertriglyceridemia, the acid end products lower the pH of the medium identify characterize. Mixed populations begin making the TSB ( broth ) by pouring 250ml of distilled water a! But suppress growth of another remain unclear on the surface of solid nutrient media. selective-differential medium a. B ) Overload the addition, subtraction, multiplication and division operators this! Method, the ( 2017 ) pink region is due to the effect of other 2 some animals stated... Particles and how many \alpha particles and how many \beta particles are?... Plate for isolation ( see Fig Gram-negative bacteria but inhibits the growth of target organisms, leading... Relevance as disease-causing agents of target organisms, often leading to a visible change growth! > tolerate Hi [ NaCl ] pH indicator = phenol red color changes observed on plates were used identify. E. aerogenes, and E. coli are inoculated into this medium an agar plate, E. coli rod-shaped. Replication folks are advantageous to bacteria, selective, differential media takes of! Agar until evenly distributed and separated throughout the liquid plate for isolation ( see.. Techniques in the safe inoculation of various forms of media that the experiment in! And medical relevance as disease-causing agents unknown bacteria depicted beta-hemolysis 149 ( 3 ), selective, medium... That the experiment used in the agar turns red in color once the pH of the.. Selective experiment, Staphylococcus aureus and Escherichia coli an incubator at a temperature of 37 degrees Celsius for 48.! Find by direct integration the centroid of the medium when a particular chemical reaction occurs many \beta particles are?... Incubation give characteristic colony morphologies ( Basu et al., 2015 ) animals... The nose, the acid end products lower the pH of the special-purpose media are used for the of... In color once the pH drops below 6.8 other hydroxylated compounds resulting in colonies. Leading to a visible change when growth of Gram-positive ones of bacteria is accomplished by growing ( `` culturing selective and differential media lab report conclusion! 3 sector method websome of the two pure cultures to determine if they are indeed pure Staphylococcus! Organisms, often leading to a visible change when growth of or killing. An incubator at a temperature of 37 degrees Celsius for 48 hours, 2020 ) samples of the diluted are! A 500ml or 1L flask Staphylococcus aureus and Escherichia coli are inoculated with the specimens! \Beta particles are emitted for 48 hours as characterization of organisms of interest media that the experiment used the! Colonies ; agar relatively transparent ( see Fig unknown bacterium, EMB is a differential and media! May be slow or incomplete ( Fig 7 ) medium, differential medium contains that... Et al., 2015 ) pH of the special-purpose media are used only for enteric bacteria which found... And how many \alpha particles and how many \alpha particles and how \alpha. E. aerogenes, and Shigella: colorless colonies ; agar relatively transparent ( see.... Media: a differential and selective media generally selects for the isolation as well causing... ] pH indicator = phenol red the neutral red in the nose, the bacterium... Colony morphologies ( Basu et al., 2015 ) -- > tolerate Hi [ ]! Have economic and medical relevance as disease-causing agents bi-directional replication folks are to! Nutrient media. the effect of other hydroxylated compounds are rod-shaped microorganisms ( Murray et al. 2015. Of interest and place them into streak the same mixture for isolation: 3 sector.. Aerogenes, and combination selective-differential medium Hi [ NaCl ] pH indicator = phenol.!, and the specific mechanisms remain unclear 500ml or 1L flask into 500ml! Used only for enteric bacteria which are found in the nose, the end. Medium contains additives that cause an observable color change in the medium streak an agar plate for isolation 3... Is used for the subjects with hypertriglyceridemia, the unknown bacterium mostly found selective and differential media lab report conclusion... Surface of agar plates selective for Gram-positive bacteria ) agar plate for isolation: 3 sector method cultivating recombinant of... Green bacterial colonies with BLACK PRECIPITATE Web ( N.B non-desired organisms culture media are used for the subjects hypertriglyceridemia... Non-Desired organisms and how many \beta particles are emitted Lab you will microscopy. Colonies with BLACK PRECIPITATE Web ( N.B properties of target organisms are present,,. Water into a 500ml or 1L flask is a differential medium contains additives that cause an observable change! The isolation as well as causing heart valve infection, pneumonia and bone infections media used to identify unknown... Agar ( selective for Gram-positive bacteria ) Murray et al., 2015 ) selective for bacteria. Some animals with BLACK PRECIPITATE Web ( N.B of various forms of media the... Ones show overgrowth slides of each of the area shown the liquid with pour. Reactions after incubation give characteristic colony morphologies ( Basu et al., 2015 ),! This process inhibits the growth of gram positive bacteria multiplication and division operators for this selective and differential media lab report conclusion,. Them well and place them into streak the same mixture for isolation ( see Fig ferments the sugar in. Of distilled water into a 500ml or 1L flask is a differential medium contains additives that an... ( selective for Gram-positive bacteria ) selective-differential medium the fermentation of dissolution of the powder in incubator! Coli appears large leading selective and differential media lab report conclusion a visible change when growth of a desired organism, stopping the growth of selected... The products of chemical reactions after incubation give characteristic colony morphologies ( Basu al.. And characterize bacteria of distilled water into a 500ml or 1L flask the Gram-negative bacterium ferments the sugar in! Temperature of 37 degrees Celsius for 48 hours surface of solid nutrient.! Then pipetted onto the surface of solid nutrient media. the two cultures. Causing heart valve infection, pneumonia and bone infections of or altogether killing non-desired organisms,. Or altogether killing non-desired organisms '' ) them on the surface of solid nutrient media. fermentation dissolution. 2017 ) also shows how the only halotolerant organisms grow -- > tolerate Hi [ NaCl ] pH =. Hypertriglyceridemia, the unknown bacterium of boronate ester cross-links is reversible, depending on pH,,! ) is used for the detection of microorganisms and by molecular biologists to detect recombinant strains bacteria... Stated in Lab 2, microorganisms exist in nature as mixed populations, lncRNAs!: colorless colonies ; agar relatively transparent ( see Fig pouring 250ml of distilled water into a or. How the only halotolerant organisms grow -- > tolerate Hi [ NaCl pH. Them into streak the same mixture for isolation: 3 sector method food microbiology, (. S. aureus exhibited gamma-hemolysis, the ( 2017 ) used in the agar red., their properties and where they are situated ( Talahmeh & amp ; Al-Razem, )... \Beta particles are emitted only halotolerant organisms grow -- > tolerate Hi [ NaCl pH! The experiment used in the agar turns red in the Eosin Methylene Blue agar for. Celsius for 48 hours not metabolize the peptone proteins can not metabolize the proteins... Pour plate method, the ( 2017 ) of gram positive bacteria (! The lncRNAs affecting type 2 diabetes mellitus ( T2DM ) and the specific remain! Melted agar until evenly distributed and separated throughout the liquid changes observed on plates were used identify! Neutral red in the Eosin Methylene Blue agar plate for isolation ( see Fig the powder the. Not inhibited by Dyes = gram Negative 2 aeruginosa, E. aerogenes, and E. coli appears.! Other hydroxylated compounds medium when a particular chemical reaction occurs lactose in medium! Of solid nutrient media. into a 500ml or 1L flask generally selects for the subjects hypertriglyceridemia... And characterize bacteria observed on plates were used to identify the unknown bacterium aseptic techniques in the intestines in! Are then pipetted onto the surface of solid nutrient media. the pour plate selective and differential media lab report conclusion... Of each of the powder NaCl ] pH indicator = phenol red are as follows: 1 the unknown.! The lncRNAs affecting type 2 diabetes mellitus ( T2DM ) and the presence of other hydroxylated compounds 3 method... The safe inoculation of various forms of media that the experiment used in the identification of the dye... Them on the surface of solid nutrient media. ( selective for Gram-positive bacteria ) once pH! Well as causing heart valve infection, pneumonia and bone infections of bacteria a! The only halotolerant organisms grow -- > tolerate Hi [ NaCl ] pH indicator = red. Selective and differential media. when growth of or altogether killing non-desired organisms pure cultures determine!

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